Intermediate

Part:BBa_K944012:Design

Designed by: Natasha Gómez   Group: iGEM12_Panama_INDICASAT   (2012-09-26)


T7 constitutive promoter with RBS, transcriptional regulator from de cyanide inducible promoter and


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 870
    Illegal NgoMIV site found at 1427
    Illegal NgoMIV site found at 1779
    Illegal AgeI site found at 1735
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 560


Design Notes

No considerations right now.


Source

Most of the parts of this intermediate construct are well characterized biobricks from the registry except for the transcriptional regulator which is a brand new biobrick we add to the registry.

References